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Simultaneous detection of alkaline phosphatase and beta-galactosidase activity using SERRS

Ingram, A.M. and Moore, B.D. and Graham, D. (2009) Simultaneous detection of alkaline phosphatase and beta-galactosidase activity using SERRS. Bioorganic and Medicinal Chemistry Letters, 19 (6). pp. 1569-1571. ISSN 0960-894X

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Abstract

Surface enhanced resonance Raman scattering (SERRS) is an alternative to fluorescence for use in bioanalysis however due to the different optical mechanism it requires specifically designed reporters. Recently we have reported the use of 8-hydroxyquinolinyl azo dyes and their ester derivatives as reporters of lipase activity using SERRS. Acylation of the 8-hydroxy moiety significantly reduces surface enhancement of the Raman response and subsequent lipase catalysed ester hydrolysis enables the analyte to bind to silver nanoparticles, thus providing surface enhancement and the SERRS signal is 'switched on'. By following this principle, phosphorylated and galactosylated analogues of 8-hydroxyquinolinylazo dyes were prepared and shown to act as reporters of enzymatic activity for alkaline phosphatase and β-galactosidase respectively when using SERRS.

Item type: Article
ID code: 16977
Keywords: enzymes, surface enhanced Raman scattering, Physical and theoretical chemistry, Biochemistry, Organic Chemistry, Drug Discovery, Pharmaceutical Science, Molecular Medicine, Molecular Biology, Clinical Biochemistry
Subjects: Science > Chemistry > Physical and theoretical chemistry
Department: Faculty of Science > Pure and Applied Chemistry
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 17 May 2010 16:25
    Last modified: 05 Sep 2014 03:22
    URI: http://strathprints.strath.ac.uk/id/eprint/16977

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