Rolinski, O.J. and Scobie, K. and Birch, D.J.S. (2009) Protein fluorescence decay : a gamma function description of thermally induced interconversion of amino acid rotamers. Physical Review E: Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics, 79 (5). ISSN 1063-651XFull text not available in this repository. (Request a copy from the Strathclyde author)
We present a description of fluorescence decay kinetics in complex environments based on gamma functions rather than the conventional approach using exponentials. The gamma function description is tested in measurements on the temperature dependence of the protein human serum albumin (HSA), N-acetyl tryptophanamide (NATA), and 2, 5-dipenyl oxazole (PPO). The monitoring of macromolecular structure and dynamics is demonstrated by means of distinct tryptophan (Trp) rotamer populations and their interconversion in HSA.
|Keywords:||fluorescence, intramolecular mechanics, macromolecules, molecular biophysics, proteins, Physics, Physics and Astronomy(all), Mathematical Physics, Statistical and Nonlinear Physics, Condensed Matter Physics|
|Subjects:||Science > Physics|
|Department:||Faculty of Science > Physics|
|Depositing user:||Strathprints Administrator|
|Date Deposited:||05 May 2010 15:01|
|Last modified:||04 May 2016 16:06|