Strathprints logo
Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Effects of eutrophication on vitellogenin gene expression in male fathead minnows (pimephales promelas) exposed to 17 alpha-ethynylestradiol in field mesocosms

Gordan, D.A. and Toth, G.P. and Graham, D.W. and Lazorchak, J.A. and Redd, T.V. and Knapp, Charles W. and deNoyelles, F. and Campbell, Scott and Lattier, D.L. (2006) Effects of eutrophication on vitellogenin gene expression in male fathead minnows (pimephales promelas) exposed to 17 alpha-ethynylestradiol in field mesocosms. Environmental Pollution, 142 (3). pp. 559-566. ISSN 0269-7491

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

This study evaluated the effect of aquatic secondary nutrient supply levels (nitrogen and phosphorus) on the subcellular response of adult male fathead minnows (Pimephales promelas) exposed to a single nominal concentration of 17 alpha-ethynytestradiol (EE2), a potent synthetic estrogen, under quasi-natural field conditions. Outdoor mesocosms were maintained under low, medium, and high nutrient supply conditions as categorized by total phosphorus (TP) level (nominal 0.012, 0.025, and 0.045 mg TP/L, respectively), and treated with EE2 with and without a carrier solvent. Using reverse transcription-polymerase chain reaction methods, vitellogenin gene (Vg) expression was determined in the fish collected at 0 h, 8 h, 24 h, 4 d, 7 d, and 14 d post-exposure. Induction of Vg was detected as early as 8 It post-exposure, with and without the carrier solvent, and persisted through Day 14. Results showed Vg to be significantly greater at low nutrient levels (p < 0.05), suggesting that EE2 bioavailability to the fish was likely greater under less-turbid water conditions. Published by Elsevier Ltd.

Item type: Article
ID code: 16346
Keywords: nutrient enrichment, 17 alpha-ethynylestradiol, fathead minnow, vitellogenin, mesocosms, Environmental engineering, Pollution, Health, Toxicology and Mutagenesis, Toxicology
Subjects: Technology > Engineering (General). Civil engineering (General) > Environmental engineering
Department: Faculty of Engineering > Civil and Environmental Engineering
Related URLs:
Depositing user: Dr Charles W Knapp
Date Deposited: 04 Mar 2010 15:00
Last modified: 05 Sep 2014 01:31
URI: http://strathprints.strath.ac.uk/id/eprint/16346

Actions (login required)

View Item