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The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including those from the School of Psychological Sciences & Health - but also papers by researchers based within the Faculties of Science, Engineering, Humanities & Social Sciences, and from the Strathclyde Business School.

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Effects of eutrophication on vitellogenin gene expression in male fathead minnows (pimephales promelas) exposed to 17 alpha-ethynylestradiol in field mesocosms

Gordan, D.A. and Toth, G.P. and Graham, D.W. and Lazorchak, J.A. and Redd, T.V. and Knapp, Charles W. and deNoyelles, F. and Campbell, Scott and Lattier, D.L. (2006) Effects of eutrophication on vitellogenin gene expression in male fathead minnows (pimephales promelas) exposed to 17 alpha-ethynylestradiol in field mesocosms. Environmental Pollution, 142 (3). pp. 559-566. ISSN 0269-7491

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Abstract

This study evaluated the effect of aquatic secondary nutrient supply levels (nitrogen and phosphorus) on the subcellular response of adult male fathead minnows (Pimephales promelas) exposed to a single nominal concentration of 17 alpha-ethynytestradiol (EE2), a potent synthetic estrogen, under quasi-natural field conditions. Outdoor mesocosms were maintained under low, medium, and high nutrient supply conditions as categorized by total phosphorus (TP) level (nominal 0.012, 0.025, and 0.045 mg TP/L, respectively), and treated with EE2 with and without a carrier solvent. Using reverse transcription-polymerase chain reaction methods, vitellogenin gene (Vg) expression was determined in the fish collected at 0 h, 8 h, 24 h, 4 d, 7 d, and 14 d post-exposure. Induction of Vg was detected as early as 8 It post-exposure, with and without the carrier solvent, and persisted through Day 14. Results showed Vg to be significantly greater at low nutrient levels (p < 0.05), suggesting that EE2 bioavailability to the fish was likely greater under less-turbid water conditions. Published by Elsevier Ltd.