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Chapter 16 two-photon microscopy and multidimensional analysis of cell dynamics

Zinselmeyer, Bernd H. and Dempster, J. and Wokosin, D.L. and Cannon, J. and Pless, Robert and Parker, Ian and Miller, Mark J. (2009) Chapter 16 two-photon microscopy and multidimensional analysis of cell dynamics. Methods in Enzmology, 461. pp. 349-378. ISSN 0076-6879

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Abstract

Two-photon (2P) microscopy is a high-resolution imaging technique that was initially applied by neurobiologists and developmental cell biologists but has subsequently been broadly adapted by immunologists. The value of 2P microscopy is that it affords an unparalleled view of single-cell spatiotemporal dynamics deep within intact tissues and organs. As the technology develops and new transgenic mice and fluorescent probes become available, 2P microscopy will serve as an increasingly valuable tool for assessing cell function and probing molecular mechanisms. Here we discuss the technical aspects related to 2P microscope design, explain in detail various tissue imaging preparations, and walk the reader through the often daunting process of analyzing multidimensional data sets and presenting the experimental results.

Item type: Article
ID code: 15317
Keywords: 2P, microscopy, single-cell spatiotemporal dynamics, cell function, probing molecular mechanisms, Therapeutics. Pharmacology, Pharmacy and materia medica, Microbiology
Subjects: Medicine > Therapeutics. Pharmacology
Medicine > Pharmacy and materia medica
Science > Microbiology
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Ms Ann Barker-Myles
    Date Deposited: 08 Feb 2010 12:41
    Last modified: 12 Mar 2012 11:01
    URI: http://strathprints.strath.ac.uk/id/eprint/15317

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