Strathprints logo
Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Protein adsorption mechanisms on solid surfaces: lysozyme-on-mica

Mulheran, P.A. and Kubiak, K. (2009) Protein adsorption mechanisms on solid surfaces: lysozyme-on-mica. Molecular Simulation, 35 (7). pp. 561-566. ISSN 0892-7022

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

A methodology for discovering the mechanisms and dynamics of protein clustering on solid surfaces is reviewed and complemented by atomistic molecular dynamics (MD) simulations. In situ atomic force microscopy images of the early stages of protein film formation are quantitatively compared with Monte Carlo simulations, using cluster statistics to differentiate various growth models. We have studied lysozyme adsorption on mica as a model system, finding that all surface-supported clusters are mobile with diffusion constant inversely related to cluster size. Furthermore, our results suggest that protein monomers diffusing to the surface from solution only adhere to the bare surface with a finite probability. Fully atomistic MD simulations reveal that the lysozyme does indeed have a preferred orientation for binding to the surface, so that proteins with incorrect orientations move away from the surface rather than towards it. Agreement with experimental studies in the literature for the residues involved in the surface adsorption is found.

Item type: Article
ID code: 15116
Keywords: biomaterials and biological interfaces, proteins, theory and modelling, computer simulation, diffusion of adsorbates, kinetics of coarsening and aggregation, Physical and theoretical chemistry, Solid state physics. Nanoscience, Modelling and Simulation, Materials Science(all), Chemical Engineering(all), Chemistry(all), Information Systems, Condensed Matter Physics
Subjects: Science > Chemistry > Physical and theoretical chemistry
Science > Physics > Solid state physics. Nanoscience
Department: Faculty of Engineering > Chemical and Process Engineering
Related URLs:
    Depositing user: Dr Paul A Mulheran
    Date Deposited: 03 Feb 2010 18:14
    Last modified: 28 Mar 2014 05:12
    URI: http://strathprints.strath.ac.uk/id/eprint/15116

    Actions (login required)

    View Item