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Mechanisms and dynamics of protein clustering on a solid surface

Mulheran, P.A. and Pellenc, D. and Bennett, R.A. and Green, R.J. and Sperrin, M. (2008) Mechanisms and dynamics of protein clustering on a solid surface. Physical Review Letters, 100 (6). ISSN 0031-9007

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Abstract

A methodology for discovering the mechanisms and dynamics of protein clustering on solid surfaces is presented. In situ atomic force microscopy images are quantitatively compared to Monte Carlo simulations using cluster statistics to differentiate various models.We study lysozyme adsorption on mica as a model system and find that all surface-supported clusters are mobile, not just the monomers, with diffusion constant inversely related to cluster size. The surface monomer diffusion constant is measured to be D-1 similar to 9x10(-16)cm(2) s(-1), such a low value being difficult to measure using other techniques.

Item type: Article
ID code: 15110
Keywords: protein clustering, mechanisms, dynamics, protein, Physical and theoretical chemistry, Solid state physics. Nanoscience, Physics and Astronomy(all)
Subjects: Science > Chemistry > Physical and theoretical chemistry
Science > Physics > Solid state physics. Nanoscience
Department: Faculty of Engineering > Chemical and Process Engineering
Related URLs:
    Depositing user: Dr Paul A Mulheran
    Date Deposited: 03 Feb 2010 16:37
    Last modified: 04 Sep 2014 22:36
    URI: http://strathprints.strath.ac.uk/id/eprint/15110

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