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Isolation of tributyltin-degrading bacteria citrobacter braakii and enterobacter cloacae from butyltin-polluted sediment

Sakultantimetha, A. and Keenan, H.E. and Dyer, M. and Beattie, T.K. and Bangkedphol, S. and Songsasen, A. (2009) Isolation of tributyltin-degrading bacteria citrobacter braakii and enterobacter cloacae from butyltin-polluted sediment. Journal of ASTM International, 6 (6). pp. 1-6. ISSN 1546-962X

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Abstract

Tributyltin compound (TBT) released into the aquatic environment is generally degraded by bacteria in water and sediment. The isolation of TBT-degrading bacteria from TBT polluted sediment leads to the indication of specific potential TBT degraders. Two new strains of bacteria designated as B2 and B3 were successfully isolated using glycerol medium containing tributyltin chloride (TBTC) at 130 μM from contaminated sediment collected from Bowling Basin in Glasgow. The observed degradation after 14 days of the microcosm from the sediment and the isolated bacteria were investigated at an initial concentration of 1 μM TBTC. It was found that TBT was degraded by the bacterial strains B2 and B3 at 8.3 and 16.9 %, respectively. The results indicate that B2 and B3 are effective as TBT degraders. EC50 of B2 and B3 in water were 88.73 and 112.53 μM TBTC, which were significantly higher than the concentration of TBT measured at the basin, suggesting a low effect of TBT on the growth and activity of bacteria. After identification using API 20E and 16S sequencing, the bacterial isolate strain B2 is Citrobacter braakii and B3 is Enterobacter cloacae. Therefore, this study has discovered two species of high resistance TBT degrader which have never been previously studied or isolated based upon TBT degradation ability.