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IGFBP-3 and IGFBP-5 associate with the cell binding domain (CBD) of fibronectin

Beattie, James and Kreiner, M. and Allan, G.J. and Flint, D.J. and Domingues, Diana and van der Walle, Christopher F. (2009) IGFBP-3 and IGFBP-5 associate with the cell binding domain (CBD) of fibronectin. Biochemical and Biophysical Research Communications, 381 (4). pp. 572-576. ISSN 1090-2104

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Abstract

We have used Surface Plasmon Resonance (SPR) - based biosensor technology to investigate the interaction of the six high affinity insulin-like growth factor binding proteins (IGFBP 1-6) with the cell binding domain (CBD) of fibronectin. Using a biotinylated derivative of the ninth and tenth TypeIII domains of FN (9-10FNIII), we show that IGFBP-3 and -5 bind to FN-CBD. We show that this binding is inhibited by IGF-I and that, for IGFBP-5, binding occurs through the C-terminal heparin binding domain of the protein. Using site-directed mutagenesis of 9-10FNIII, we show both the 'synergy' and RGD sites within these FN domains are required for maximum binding of both IGFBPs. We discuss the possible biological consequences of our results.

Item type: Article
ID code: 13216
Keywords: fibronectin, insulin-like growth factor binding protein, surface plasmon resonance, pharmacology, Therapeutics. Pharmacology, Pharmacy and materia medica, Microbiology
Subjects: Medicine > Therapeutics. Pharmacology
Medicine > Pharmacy and materia medica
Science > Microbiology
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Ms Ann Barker-Myles
    Date Deposited: 13 Oct 2009 10:46
    Last modified: 16 Jul 2013 23:15
    URI: http://strathprints.strath.ac.uk/id/eprint/13216

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