Strathprints logo
Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Pulsed electric field as a potential new method for microbial inactivation in scaffold materials for tissue engineering : the effect on collagen as a scaffold

Smith, Sharon and Griffiths, S. and MacGregor, Scott and Beveridge, Joe and Anderson, John and van der Walle, Christopher F. and Grant, M.H. (2009) Pulsed electric field as a potential new method for microbial inactivation in scaffold materials for tissue engineering : the effect on collagen as a scaffold. Journal of Biomedical Materials Research Part A, 90A (3). pp. 844-851. ISSN 1549-3296

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

Hybrid scaffolds for tissue engineering are becoming increasingly complex through incorporation of biologically active biomacromolecules. There is a need to develop a compatible sterilization method that is capable of killing microorganisms, without adversely affecting the labile scaffold biomaterials or biomacromolecular components. Pulsed electric field (PEF) treatment has been successful as a nonthermal microbial inactivation-pasteurization method within the food industry. We have previously demonstrated that PEF treatment inactivates E. coli seeded in collagen gels. Here, we show that PEF treatment does not affect the structural integrity of the collagen molecule or its adsorption to polystyrene and hydroxyapatite surfaces. Moreover, osteoblast cells cultured on PEF-treated collagen, which was coated onto two- and three-dimensional scaffolds, retained their normal morphology, growth rate, and functionality. PEF treatment, therefore, shows great potential to be used as a sterilization method for collagen-based biomaterials.

Item type: Article
ID code: 13167
Keywords: pulsed electric field, sterilization of biomaterials, scaffolds for tissue engineering, collagen, cell culture, Therapeutics. Pharmacology, Pharmacy and materia medica, Microbiology, Medicine(all)
Subjects: Medicine > Therapeutics. Pharmacology
Medicine > Pharmacy and materia medica
Science > Microbiology
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Faculty of Engineering > Bioengineering
Faculty of Engineering > Electronic and Electrical Engineering
Related URLs:
    Depositing user: Ms Ann Barker-Myles
    Date Deposited: 07 Oct 2009 11:02
    Last modified: 04 Sep 2014 22:51
    URI: http://strathprints.strath.ac.uk/id/eprint/13167

    Actions (login required)

    View Item