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The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs.

Strathprints serves world leading Open Access research by the University of Strathclyde, including research by the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), where research centres such as the Industrial Biotechnology Innovation Centre (IBioIC), the Cancer Research UK Formulation Unit, SeaBioTech and the Centre for Biophotonics are based.

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A methodology for investigating protein adhesion and adsorption to microarrayed combinatorial libraries

Taylor, Michael and Urquhart, Andrew J. and Anderson, Daniel G. and Williams, Philip M. and Langer, Robert S. and Alexander, Morgan R. and Davies, Martyn C. (2008) A methodology for investigating protein adhesion and adsorption to microarrayed combinatorial libraries. Macromolecular Rapid Communications, 29 (15). pp. 1298-1302. ISSN 1022-1336

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Abstract

Here, we report the use of fluorescently labelled proteins to study protein adsorption to microarrayed synthetic polymers for the first time, indicating that this method is appropriate for the study of protein adsorption on these arrays. To investigate protein adhesion directly we use atomic force microscopy (AFM) to measure the force of adhesion between a protein-coated probe and the arrayed polymers. Both approaches show promise as methods for screening protein interactions with polymers in a microarray format. Comparison of these very different measures of protein-surface interactions indicate a good correlation.