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A methodology for investigating protein adhesion and adsorption to microarrayed combinatorial libraries

Taylor, Michael and Urquhart, Andrew J. and Anderson, Daniel G. and Williams, Philip M. and Langer, Robert S. and Alexander, Morgan R. and Davies, Martyn C. (2008) A methodology for investigating protein adhesion and adsorption to microarrayed combinatorial libraries. Macromolecular Rapid Communications, 29 (15). pp. 1298-1302. ISSN 1022-1336

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Abstract

Here, we report the use of fluorescently labelled proteins to study protein adsorption to microarrayed synthetic polymers for the first time, indicating that this method is appropriate for the study of protein adsorption on these arrays. To investigate protein adhesion directly we use atomic force microscopy (AFM) to measure the force of adhesion between a protein-coated probe and the arrayed polymers. Both approaches show promise as methods for screening protein interactions with polymers in a microarray format. Comparison of these very different measures of protein-surface interactions indicate a good correlation.

Item type: Article
ID code: 13155
Keywords: adsorption, high-throughput screening, microarray, polymer library, proteins, Therapeutics. Pharmacology, Pharmacy and materia medica, Microbiology, Materials Chemistry, Organic Chemistry, Polymers and Plastics
Subjects: Medicine > Therapeutics. Pharmacology
Medicine > Pharmacy and materia medica
Science > Microbiology
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Ms Ann Barker-Myles
    Date Deposited: 06 Oct 2009 11:47
    Last modified: 04 Sep 2014 22:48
    URI: http://strathprints.strath.ac.uk/id/eprint/13155

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