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World class computing and information science research at Strathclyde...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including by researchers from the Department of Computer & Information Sciences involved in mathematically structured programming, similarity and metric search, computer security, software systems, combinatronics and digital health.

The Department also includes the iSchool Research Group, which performs leading research into socio-technical phenomena and topics such as information retrieval and information seeking behaviour.

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A methodology for investigating protein adhesion and adsorption to microarrayed combinatorial libraries

Taylor, Michael and Urquhart, Andrew J. and Anderson, Daniel G. and Williams, Philip M. and Langer, Robert S. and Alexander, Morgan R. and Davies, Martyn C. (2008) A methodology for investigating protein adhesion and adsorption to microarrayed combinatorial libraries. Macromolecular Rapid Communications, 29 (15). pp. 1298-1302. ISSN 1022-1336

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Abstract

Here, we report the use of fluorescently labelled proteins to study protein adsorption to microarrayed synthetic polymers for the first time, indicating that this method is appropriate for the study of protein adsorption on these arrays. To investigate protein adhesion directly we use atomic force microscopy (AFM) to measure the force of adhesion between a protein-coated probe and the arrayed polymers. Both approaches show promise as methods for screening protein interactions with polymers in a microarray format. Comparison of these very different measures of protein-surface interactions indicate a good correlation.