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Influence of parasite encoded inhibitors of serine peptidases in early infection of macrophages with Leishmania major

Eschenlauer, Sylvain C P and Faria, Marilia S and Morrison, Lesley S and Bland, Nicolas and Ribeiro-Gomes, Flavia L and DosReis, George A and Coombs, Graham H and Lima, Ana Paula C A and Mottram, Jeremy C (2008) Influence of parasite encoded inhibitors of serine peptidases in early infection of macrophages with Leishmania major. Cellular Microbiology, 11 (1). pp. 106-120. ISSN 1462-5814

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Abstract

Ecotin is a potent inhibitor of family S1A serine peptidases, enzymes lacking in the protozoan parasite Leishmania major. Nevertheless, L. major has three ecotin-like genes, termed inhibitor of serine peptidase (ISP). ISP1 is expressed in vector-borne procyclic and metacyclic promastigotes, whereas ISP2 is also expressed in the mammalian amastigote stage. Recombinant ISP2 inhibited neutrophil elastase, trypsin and chymotrypsin with Kis between 7.7 and 83 nM. L. major ISP2-ISP3 double null mutants (-isp2/3) were created. These grew normally as promastigotes, but were internalized by macrophages more efficiently than wild-type parasites due to the upregulation of phagocytosis by a mechanism dependent on serine peptidase activity. -isp2/3 promastigotes transformed to amastigotes, but failed to divide for 48 h. Intracellular multiplication of -isp2/3 was similar to wild-type parasites when serine peptidase inhibitors were present, suggesting that defective intracellular growth results from the lack of serine peptidase inhibition during promastigote uptake. -isp2/3 mutants were more infective than wild-type parasites to BALB/c mice at the early stages of infection, but became equivalent as the infection progressed. These data support the hypothesis that ISPs of L. major target host serine peptidases and influence the early stages of infection of the mammalian host.

Item type: Article
ID code: 12964
Keywords: parasite, inhibitors, serine peptidases, infections, macrophages, Leishmania, pharmacology, Therapeutics. Pharmacology, Pharmacy and materia medica, Microbiology, Immunology, Virology, Microbiology
Subjects: Medicine > Therapeutics. Pharmacology
Medicine > Pharmacy and materia medica
Science > Microbiology
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Ms Ann Barker-Myles
    Date Deposited: 27 Aug 2009 11:03
    Last modified: 04 Sep 2014 23:00
    URI: http://strathprints.strath.ac.uk/id/eprint/12964

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