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Mapping the interactions among biomaterials, adsorbed proteins, and human embryonic stem cells

Mei, Ying and Gerecht, Sharon and Taylor, Michael and Urquhart, Andrew J. and Bogatyrev, Said R. and Cho, Seung-Woo and Davies, Martyn C. and Alexander, Morgan R. and Langer, Robert S. and Anderson, Daniel G. (2009) Mapping the interactions among biomaterials, adsorbed proteins, and human embryonic stem cells. Advanced Materials, 21 (27). pp. 2781-2786. ISSN 1521-4095

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Abstract

An integrated high-throughput polymer synthesis and rapid material/protein/cell interaction assays were developed to optimize stem cell microenvironments. Microarrayed polymers were synthesized and studied for the ability to support the growth of partially differentiated human embryonic stem cells. In parallel, a programmed laser scanning cytometry system was developed to allow for rapid quantification of cell material interaction.