McConnell, G. (2005) Noise analysis of a white-light supercontinuum light source for multiple wavelength confocal laser scanning fluorescence microscopy. Journal of Physics D: Applied Physics, 38 (15). pp. 2620-2624. ISSN 0022-3727
Full text not available in this repository. (Request a copy from the Strathclyde author)Abstract
Intensity correlations of a Ti : sapphire, Kr/Ar and a white-light supercontinuum were performed to quantify the typical signal amplitude fluctuations and hence ascertain the comparative output stability of the white-light supercontinuum source for confocal laser scanning microscopy (CLSM). Intensity correlations across a two-pixel sample (n = 1000) of up to 98%, 95% and 94% were measured for the Ti : sapphire, Kr/Ar and white-light supercontinuum source, respectively. The white-light supercontinuum noise level is therefore acceptable for CLSM, with the added advantage of wider wavelength flexibility over traditional CLSM excitation sources. The relatively low-noise white-light supercontinuum was then used to perform multiple wavelength sequential CLSM of guinea pig detrusor to confirm the reliability of the system and to demonstrate system flexibility.
| Item type: | Article |
|---|---|
| ID code: | 119 |
| Keywords: | signal amplitude fluctuations, white-light supercontinuum, CLSM excitation sources, guinea pig detrusor, signal amplitude fluctuations, Optics. Light |
| Subjects: | Science > Physics > Optics. Light |
| Department: | Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences |
| Related URLs: | |
| Depositing user: | Ms FM Breslin |
| Date Deposited: | 13 Feb 2006 |
| Last modified: | 04 Oct 2012 11:56 |
| URI: | http://strathprints.strath.ac.uk/id/eprint/119 |
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