Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Detection of DNA probes using Diels Alder cycloaddition and SERRS

Graham, D. and Fruk, L. and Smith, W.E. (2003) Detection of DNA probes using Diels Alder cycloaddition and SERRS. Analyst, 128 (6). pp. 692-699. ISSN 0003-2654

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

A number of methods for detecting specific DNA sequences have been used to provide data for use in diagnosis of disease states and examination of gene expression. This study shows how the use of labelled oligonucleotides created by Diels Alder cycloaddition can be used as surface enhanced resonance Raman scattering (SERRS) active probes that provide easily identifiable signals at low concentrations in a mixture. The probes were produced by first tagging the oligonucleotides with a furan residue at the 5'-terminus to act as the diene. Three specifically designed benzotriazole azo maleimide dyes were then used as dienophiles to undergo cycloaddition with the furan modified oligonucleotide to generate SERRS active probes. These probes gave excellent SERRS signals from a silver-PVA film. Surface mapping of the silver PVA film indicated that the distribution of the dyes was uniform and that the number of moles of probe detected at any one time was approximately in the attomole region.

Item type: Article
ID code: 1100
Keywords: resonance-raman-scattering, surface-enhanced raman, single-molecule detection, benzotriazole azo-dye, colloidal silver, human genome, labeled dna, oligonucleotides, acid, Chemistry
Subjects: Science > Chemistry
Department: Faculty of Science > Pure and Applied Chemistry
Related URLs:
Depositing user: Mr Derek Boyle
Date Deposited: 18 May 2006
Last modified: 12 Mar 2012 10:36
URI: http://strathprints.strath.ac.uk/id/eprint/1100

Actions (login required)

View Item