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Capillary electrophoresis for studying drug-DNA interactions

Araya, F.G. and Huchet, G. and McGroarty, I. and Skellern, G.G. and Waigh, R.D. (2007) Capillary electrophoresis for studying drug-DNA interactions. Methods, 42 (2). pp. 141-149. ISSN 1046-2023

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Abstract

The development of new drugs to treat disease by binding directly to DNA offers much promise but is reliant on methods to determine the relative affinity of the putative drug for different DNA sequences. Such methods should ideally be rapid and inexpensive as well as reliable. Use of capillary electrophoresis in simple silica columns offers such a method. The development of systems in which the solvent carries a soluble polymer allows the reliable separation of DNA oligomers, of 12–20 bp in length, which can then be titrated with the ligand in competition experiments. The results obtained are comparable with those obtained by footprinting and give direct graphical output, easily analysed for relative binding affinity.

Item type: Article
ID code: 10988
Keywords: capillary electrophoresis, DNA interaction, DNA, DNA oligomers , ligand binding, Pharmacy and materia medica, Therapeutics. Pharmacology
Subjects: Medicine > Pharmacy and materia medica
Medicine > Therapeutics. Pharmacology
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 16 Nov 2011 10:47
    Last modified: 06 Dec 2013 19:56
    URI: http://strathprints.strath.ac.uk/id/eprint/10988

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