Araya, F.G. and Huchet, G. and McGroarty, I. and Skellern, G.G. and Waigh, R.D. (2007) Capillary electrophoresis for studying drug-DNA interactions. Methods, 42 (2). pp. 141-149. ISSN 1046-2023
Full text not available in this repository. (Request a copy from the Strathclyde author)Abstract
The development of new drugs to treat disease by binding directly to DNA offers much promise but is reliant on methods to determine the relative affinity of the putative drug for different DNA sequences. Such methods should ideally be rapid and inexpensive as well as reliable. Use of capillary electrophoresis in simple silica columns offers such a method. The development of systems in which the solvent carries a soluble polymer allows the reliable separation of DNA oligomers, of 12–20 bp in length, which can then be titrated with the ligand in competition experiments. The results obtained are comparable with those obtained by footprinting and give direct graphical output, easily analysed for relative binding affinity.
| Item type: | Article |
|---|---|
| ID code: | 10988 |
| Keywords: | capillary electrophoresis, DNA interaction, DNA, DNA oligomers , ligand binding, Pharmacy and materia medica, Therapeutics. Pharmacology |
| Subjects: | Medicine > Pharmacy and materia medica Medicine > Therapeutics. Pharmacology |
| Department: | Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences |
| Related URLs: | |
| Depositing user: | Strathprints Administrator |
| Date Deposited: | 16 Nov 2011 10:47 |
| Last modified: | 12 Mar 2012 10:54 |
| URI: | http://strathprints.strath.ac.uk/id/eprint/10988 |
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