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Immunomodulatory dendritic cells in intestinal lamina propria

Chirdo, F.G. and Millington, O.R. and Beacock-Sharp, H. and Mowat, A.M. (2005) Immunomodulatory dendritic cells in intestinal lamina propria. European Journal of Immunology, 35 (6). pp. 1831-1840. ISSN 0014-2980

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The lamina propria (LP) of the small intestine contains many dendritic cells (DC), which are likely to be in close contact with luminal antigens, but their role in intestinal immune responses has been overlooked. Here we show that after feeding mice ovalbumin (OVA), the majority of antigen uptake is associated with DC in the small intestinal LP, and we describe the isolation, purification and initial characterization of theses DC. We obtained >90% CD11c+ DC using magnetic cell sorting, of which the majority were CD11b+CD8–, with smaller numbers of CD11b–CD8+ and CD11b–CD8– DC as well as a distinct population of CD11cintclass II MHClo B220+ DC. Freshly isolated LP DC expressed variable but generally low levels of CD40, CD80 and CD86, which were up-regulated by activation with LPS. LP DC were endocytic in vivo and in vitro and could present antigen to OVA-specific CD4+ T cells in vitro. Antigen-loaded LP DC from OVA-fed mice also primed specific CD4+ T cells in vivo and in vitro, but adoptive transfer of these DC into naive recipients induced hyporesponsiveness to subsequent challenge. LP DC also expressed significant levels of mRNA for IL-10 and type I IFN, but not IL-12, suggesting they may play a central and unique role in immune homeostasis in the gut.

Item type: Article
ID code: 10984
Keywords: Dendritic cells, oral tolerance, Lamina propria, Intestinal mucosa, Pharmacy and materia medica, Immunology, Immunology and Allergy
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Depositing user: Strathprints Administrator
Date Deposited: 23 Jun 2011 13:32
Last modified: 10 Dec 2015 17:44

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