Khan, M.A.H. and Ferro, V.A. and Koyama, M. and Kinugasa, Y. and Song, M. and Ogita, K. and Murata, Y. and Kimura, T. (2007) Immunisation of male mice with a plasmid DNA vaccine encoding gonadotrophin releasing hormone (GnRH-I) and t-helper epitopes suppresses fertility in vivo. Vaccine, 25 (18). pp. 3544-3553. ISSN 0264-410XFull text not available in this repository. (Request a copy from the Strathclyde author)
Immunisation against mammalian gonadotrophin releasing hormone (GnRH-I) linked to large carrier proteins has been shown to disrupt fertility. However, various studies have shown that the carrier protein causes epitope suppression of the hapten response, resulting in short-lived immunoneutralisation, followed by a return of fertility. A range of strategies has been used to resolve this, with limited success. The aim of this study was to construct a plasmid DNA vaccine encoding GnRH-I and T-helper epitopes. A 498 bp long vaccine construct in pcDNA3.1+ was administered to male mice in conjunction with a Hemagglutinating Virus of Japanese Envelop (HVJ-E) vector or in saline solution. The vaccine efficacy was evaluated in terms of GnRH-I specific IgG antibody response, serum testosterone levels, testicular spermatogenesis and the ability to produce offspring. The vaccine appeared to induce higher anti-GnRH-I IgG antibody response and insult the fertility axis, which was characterised by a drop of epididymal sperm counts, reduction of serum testosterone levels, suppressed testicular spermatogenesis and a significant decrease in litter numbers compared to control animals. The end-point vaccine efficacy was much higher in the HVJ-E vector mediated immunisation, than in saline alone.
|Keywords:||in vivo fertility, anti-GnRH-I antibody, GnRH-I vaccine, Pharmacy and materia medica, Infectious Diseases, Molecular Medicine, Public Health, Environmental and Occupational Health, veterinary(all), Immunology and Microbiology(all)|
|Subjects:||Medicine > Pharmacy and materia medica|
|Department:||Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences|
|Depositing user:||Strathprints Administrator|
|Date Deposited:||27 Jul 2011 09:05|
|Last modified:||13 May 2016 03:08|