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Capillary electrochromatography of therapeutic peptides on mixed-mode butylmethacrylate monoliths

Adu, J.K. and Lau, S. and Watson, D.G. and Euerby, M.R. and Skellern, G.G. and Tettey, J.N.A. (2005) Capillary electrochromatography of therapeutic peptides on mixed-mode butylmethacrylate monoliths. Electrophoresis, 26 (18). pp. 3445-3451. ISSN 0173-0835

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Abstract

In this study, a porous mixed-mode n-alkyl methacrylate-based monolith has been used in the separation of therapeutic peptides. While the sulfonic acid (SCX) moiety derived from 2-acrylamido-2-methyl-1-propanesulfonic acid supports the generation of a stable electroosmotic flow (EOF) at both acidic and basic pH values, the butyl ligands provide the nonpolar sites for chromatographic resolution. The performance of the monolith was evaluated regarding the influence of pH on chromatographic resolution of peptides. The suitability of the butylmethacrylate/SCX monolith for the analysis of therapeutic peptides containing basic centres, for example arginine, at moderately high pH 9.5 and the stability to repeat injections of a mixture of peptides was demonstrated. Separations with efficiencies as high as 5.06105 plates/m were obtained and the migration behaviour of the peptides at both low (2.8) and high (9.5) pH values could be rationalised based on their charge, molecular mass/shape and relative hydrophobicities.

Item type: Article
ID code: 10604
Keywords: capillary electrochromatography, monolithic stationary phases, therapeutic peptides, Pharmacy and materia medica, Biochemistry, Clinical Biochemistry
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Unknown Department
Depositing user: Strathprints Administrator
Date Deposited: 01 Oct 2010 18:01
Last modified: 21 May 2015 10:19
URI: http://strathprints.strath.ac.uk/id/eprint/10604

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