Adu, J.K. and Lau, S. and Watson, D.G. and Euerby, M.R. and Skellern, G.G. and Tettey, J.N.A. (2005) Capillary electrochromatography of therapeutic peptides on mixed-mode butylmethacrylate monoliths. Electrophoresis, 26 (18). pp. 3445-3451. ISSN 0173-0835Full text not available in this repository. (Request a copy from the Strathclyde author)
In this study, a porous mixed-mode n-alkyl methacrylate-based monolith has been used in the separation of therapeutic peptides. While the sulfonic acid (SCX) moiety derived from 2-acrylamido-2-methyl-1-propanesulfonic acid supports the generation of a stable electroosmotic flow (EOF) at both acidic and basic pH values, the butyl ligands provide the nonpolar sites for chromatographic resolution. The performance of the monolith was evaluated regarding the influence of pH on chromatographic resolution of peptides. The suitability of the butylmethacrylate/SCX monolith for the analysis of therapeutic peptides containing basic centres, for example arginine, at moderately high pH 9.5 and the stability to repeat injections of a mixture of peptides was demonstrated. Separations with efficiencies as high as 5.06105 plates/m were obtained and the migration behaviour of the peptides at both low (2.8) and high (9.5) pH values could be rationalised based on their charge, molecular mass/shape and relative hydrophobicities.
|Keywords:||capillary electrochromatography, monolithic stationary phases, therapeutic peptides, Pharmacy and materia medica, Biochemistry, Clinical Biochemistry|
|Subjects:||Medicine > Pharmacy and materia medica|
|Department:||Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
|Depositing user:||Strathprints Administrator|
|Date Deposited:||01 Oct 2010 18:01|
|Last modified:||06 Jan 2017 06:25|