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Strathprints serves world leading Open Access research by the University of Strathclyde, including research by the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), where research centres such as the Industrial Biotechnology Innovation Centre (IBioIC), the Cancer Research UK Formulation Unit, SeaBioTech and the Centre for Biophotonics are based.

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Indoleamine 2,3-dioxygenase activity and L-tryptophan transport in human breast cancer cells

Travers, M. and Gow, Iain F. and Barber, M.C. and Thomson, Jean and Shennan, David B. (2004) Indoleamine 2,3-dioxygenase activity and L-tryptophan transport in human breast cancer cells. BBA - Biomembranes, 1661 (1). pp. 106-112. ISSN 0005-2736

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Abstract

The activity and expression of indoleamine 2,3-dioxygenase together with -tryptophan transport has been examined in cultured human breast cancer cells. MDA-MB-231 but not MCF-7 cells expressed mRNA for indoleamine 2,3-dioxygenase. Kynurenine production by MDA-MB-231 cells, which was taken as a measure of enzyme activity, was markedly stimulated by interferon-γ (1000 units/ml). Accordingly, -tryptophan utilization by MDA-MB-231 cells was enhanced by interferon-γ. 1-Methyl- -tryptophan (1 mM) inhibited interferon-γ induced kynurenine production by MBA-MB-231 cells. Kynurenine production by MCF-7 cells remained at basal levels when cultured in the presence of interferon-γ. -Tryptophan transport into MDA-MB-231 cells was via a Na+-independent, BCH-sensitive pathway. It appears that system L (LAT1/CD98) may be the only pathway for -tryptophan transport into these cells. 1-Methyl- , -tryptophan trans-stimulated -tryptophan efflux from MDA-MB-231 cells and thus appears to be a transported substrate of system L. The results suggest that system L plays an important role in providing indoleamine-2,3-dioxygenase with its main substrate, -tryptophan, and suggest a mechanism by which estrogen receptor-negative breast cancer cells may evade the attention of the immune system.