MacKenzie, C. and Ritchie, E. and Paul, A. and Plevin, R.J. (2007) IKKalpha and IKKbeta function in TNFalpha-stimulated adhesion molecule expression in human aortic smooth muscle cells. Cellular Signalling, 19. pp. 75-80. ISSN 0898-6568Full text not available in this repository. (Request a copy from the Strathclyde author)
The role of NFκB and it's upstream kinases in regulating adhesion molecule expression in the smooth muscle of the vasculature remains controversial. We therefore examined the effect of blocking the NFκB pathway on TNFα-stimulated ICAM-1 and VCAM-1 expression in primary cultures of human aortic smooth muscle cells using an adenoviral wild-type IκBα construct (Ad.IκBα) and dominant-negative IKKα (Ad.IKKα+/−) and IKKβ (Ad.IKKβ+/−) constructs. Ad.IκBα treatment was found to block NFκB DNA-binding, and thereby completely prevent TNFα-stimulated ICAM-1 and VCAM-1 expression without influencing IKK activity. Ad.IKKβ+/− treatment completely inhibited TNFα-stimulated IKK kinase activity, IκBα degradation and NFκB DNA-binding in addition to completely blocking TNFα-stimulated ICAM-1 and VCAM-1 expression. Ad.IKKα+/− treatment however had no detectable effect on NFκB DNA-binding or ICAM-1 and VCAM-1 expression. Our results demonstrate that TNFα-stimulated ICAM-1 and VCAM-1 expression in human aortic smooth muscle cells is NFκB-dependent, that IKKβ is a suitable target for drug therapy and Ad.IKKβ+/− an effective inhibitor of TNFα-stimulated ICAM-1 and VCAM-1 expression.
|Keywords:||human aortic smooth muscle cells , vascular cell adhesion molecule-1, intercellular adhesion molecule-1 , nuclear factor kappa B, inhibitory kappa B kinase , Pharmacy and materia medica, Cell Biology|
|Subjects:||Medicine > Pharmacy and materia medica|
|Department:||Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences|
|Depositing user:||Strathprints Administrator|
|Date Deposited:||05 Dec 2011 14:16|
|Last modified:||06 Jan 2017 06:35|