Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

At-line monitoring of ammonium, glucose, methyl oleate and biomass in a complex antibiotic fermentation process using attenuated total reflectance-mid-infrared (ATR-MIR) spectroscopy

Roychoudhury, P. and Harvey, L.M. and McNeil, B. (2006) At-line monitoring of ammonium, glucose, methyl oleate and biomass in a complex antibiotic fermentation process using attenuated total reflectance-mid-infrared (ATR-MIR) spectroscopy. Analytica Chimica Acta, 561 (1-2). pp. 218-224. ISSN 0003-2670

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

The utility of attenuated total reflectance-mid-infrared spectroscopy (ATR-MIR) to monitor the concentrations of the key analytes ammonium, glucose, methyl oleate and biomass in a Streptomyces clavuligerus bioprocess for the synthesis of clavulanic acid was investigated. The cultivation medium was complex and the process fluid (matrix) underwent profound changes as culture growth proceeded, representing a considerable challenge from a spectroscopic viewpoint. Quantitative models were developed using the multivariate statistical technique, partial least square (PLS) for the key analytes over the entire time course of the bioprocess, which were validated externally using samples not incorporated in the original modelling exercise. The reasoning behind the choice of modelling strategy for each analyte is discussed, with particular focus on biomass modelling in order to generate clearer insight into the modelling process in this complex matrix. Despite the heterogeneous nature of the sample matrix, and the complexity of the spectral information arising, at-line models were developed giving low prediction error values for the analytes: ammonium 0.013 g/l; glucose 0.56 g/l; methyl oleate 0.38 g/l and biomass 0.39 g/l, respectively. These findings represent a considerable advance on previous studies using NIR in antibiotic processes, as all key analytes have been successfully modelled here, and the use of ATR-MIR in complex bioprocess fluids has been shown to be a viable method of near real-time monitoring.

Item type: Article
ID code: 10526
Keywords: ATR-MIR, streptomyces, PLS, models, Pharmacy and materia medica
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Unknown Department
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 07 Dec 2011 16:15
    Last modified: 04 Oct 2012 12:34
    URI: http://strathprints.strath.ac.uk/id/eprint/10526

    Actions (login required)

    View Item