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The effect of a hyposmotic shock and purinergic agonists on K+(Rb+) efflux from cultured human breast cancer cells

Gow, I.F. and Thomson, Jean and Davidson, Jillian and Shennan, D.B. (2005) The effect of a hyposmotic shock and purinergic agonists on K+(Rb+) efflux from cultured human breast cancer cells. BBA - Biomembranes, 1712 (1). pp. 52-61. ISSN 0005-2736

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Abstract

The effect of a hyposmotic shock and extracellular ATP on the efflux of K+(Rb+) from human breast cancer cell lines (MDA-MB-231 and MCF-7) has been examined. A hyposmotic shock increased the fractional efflux of K+(Rb+) from MDA-MB-231 cells via a pathway which was unaffected by Cl− replacement. Apamin, charybdotoxin or removing extracellular Ca2+ had no effect on volume-activated K+(Rb+) efflux MDA-MB-231 cells. An osmotic shock also stimulated K+(Rb+) efflux from MCF-7 cells but to a much lesser extent than found with MDA-MB-231 cells. ATP-stimulated K+(Rb+) efflux from MDA-MB-231 cells in a dose-dependent fashion but had little effect on K+(Rb+) release from MCF-7 cells. ATP-stimulated K+(Rb+) efflux was only inhibited slightly by replacing Cl− with NO3−. Removal of external Ca2+ during treatment with ATP reduced the fractional efflux of K+(Rb+) in a manner suggesting a role for cellular Ca2+ stores. Charybdotoxin, but neither apamin nor iberiotoxin, inhibited ATP-stimulated K+(Rb+) release from MDA-MB-231 cells. Suramin inhibited the ATP-activated efflux of K+(Rb+). UTP also stimulated K+(Rb+) efflux from MDA-MB-231 cells whereas ADP, AMP and adenosine were without effect. A combination of an osmotic shock and ATP increased the fractional efflux of K+(Rb+) to a level greater than the sum of the individual treatments. It appears that the hyposmotically-activated and ATP-stimulated K+ efflux pathways are separate entities. However, there may be a degree of 'crosstalk' between the two pathways.

Item type: Article
ID code: 10480
Keywords: hyposmotic shock, purinergic agonist, breast cancer cell, Pharmacy and materia medica, Biochemistry, Cell Biology, Biophysics
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 12 May 2010 11:45
    Last modified: 04 Sep 2014 19:55
    URI: http://strathprints.strath.ac.uk/id/eprint/10480

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