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Strathprints serves world leading Open Access research by the University of Strathclyde, including research by the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), where research centres such as the Industrial Biotechnology Innovation Centre (IBioIC), the Cancer Research UK Formulation Unit, SeaBioTech and the Centre for Biophotonics are based.

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Properties of the cellular prion protein expressed in Xenopus oocytes

Connolly, John G. and Tate, Roth J. and McLennan, Neil F. and Brown, Debbie and Telling, Glenn C. and Fraser, Jan and Head, Mark W. (2002) Properties of the cellular prion protein expressed in Xenopus oocytes. NeuroReport, 13 (9). pp. 1229-1233. ISSN 0959-4965

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Abstract

The cellular prion protein (PrPC) from different species can be reproducibly expressed in Xenopus oocytes following injection of in vitro transcribed mRNAs. The level of PrPC accumulation increases with the amount of RNA injected and with the duration of incubation. PrPC expressed in oocytes is similar in size and abundance to PrPC: protein in mouse brain and > 100 ng of PrPC is expressed per oocyte allowing complete experiments to be carried out in single living cells. The protein is glycosylated, fully protease sensitive and expressed on the cell surface. Xenopus oocytes therefore provide a useful model system for the study of prion proteins and their associated disease processes.