Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Asymmetric expression of transcripts derived from the shared promoter between the divergently orientated aCACA and tADA2L genes

Travers, M. and Cambot, M. and Kennedy, H.T. and Lenoir, G.M. and Barber, M.C. and Joulin, V. (2005) Asymmetric expression of transcripts derived from the shared promoter between the divergently orientated aCACA and tADA2L genes. Genomics, 85 (1). pp. 71-84. ISSN 1089-8646

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

The mammalian gene (ACACA) encoding acetyl-CoA carboxylase-α, a key regulatory enzyme of fatty acid synthesis, is transcribed from multiple promoters. We have delineated the 5′ boundary of ACACA in four species (human, mouse, rat, and ovine). The 5′ end of ACACA is located within a 600- to 700-bp CpG island encompassing a bidirectional promoter shared with the divergently oriented TADA2L, which encodes a component of chromatin-modifying complexes. In mouse and rat, this promoter, now referred to as Acaca PI, is located 43 kb upstream of the previously known regulatory regions. The shared promoter coregulates transcripts for TADA2L and ACACA in an asymmetric fashion in human and mouse tissues. A higher concentration of RNA polymerase II (Pol II) within the intergenic region in brain compared to liver of mouse reflects the greater abundance of the two transcripts in brain. The concentration of Pol II tracking downstream, which is lower than at the promoter, is not significantly different in either gene in the two tissues and does not reflect the 10- and >200-fold greater abundance of Tada2l and Acaca PI transcripts, respectively, in brain. Thus, regulation of clearance of Pol II from the promoter and the rate of elongation may therefore be determinants of the asymmetric expression of these transcripts.

Item type: Article
ID code: 10440
Keywords: ACC1, SAGA-type complexes, histone acetylation, bidirectional promoters, adipose tissue, brain , mouse, ovine, human, Isozyme, Lipogenesis, Pharmacy and materia medica
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 14 Nov 2011 10:44
    Last modified: 16 Jul 2013 21:49
    URI: http://strathprints.strath.ac.uk/id/eprint/10440

    Actions (login required)

    View Item