Duffy, D. and Yang, C.P. and Heath, A. and Garside, P. and Bell, E.B. (2006) Naive t-cell receptor transgenic t cells help memory b cells produce antibody. Immunology, 119. pp. 376-384. ISSN 0019-2805Full text not available in this repository. (Request a copy from the Strathclyde author)
Injection of the same antigen following primary immunization induces a classic secondary response characterized by a large quantity of high-affinity antibody of an immunoglobulin G class produced more rapidly than in the initial response – the products of memory B cells are qualitatively distinct from that of the original naive B lymphocytes. Very little is known of the help provided by the CD4 T cells that stimulate memory B cells. Using antigen-specific T-cell receptor transgenic CD4 T cells (DO11.10) as a source of help, we found that naive transgenic T cells stimulated memory B cells almost as well (in terms of quantity and speed) as transgenic T cells that had been recently primed. There was a direct correlation between serum antibody levels and the number of naive transgenic T cells transferred. Using T cells from transgenic interleukin-2-deficient mice we showed that interleukin-2 was not required for a secondary response, although it was necessary for a primary response. The results suggested that the signals delivered by CD4 T cells and required by memory B cells for their activation were common to both antigen-primed and naive CD4 T cells.
|Keywords:||adoptive transfer, transgenic, memory, CD45RB, CD4 T cells, Pharmacy and materia medica, Immunology, Immunology and Allergy|
|Subjects:||Medicine > Pharmacy and materia medica|
|Department:||Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences|
|Depositing user:||Strathprints Administrator|
|Date Deposited:||04 Oct 2011 09:25|
|Last modified:||22 Mar 2017 10:02|