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Molecularly imprinted solid-phase extraction of diazepam and its metabolites from hair samples

Cormack, P.A.G. and Ariffin, M.M. and Miller, E.I. and Anderson, R.A. (2007) Molecularly imprinted solid-phase extraction of diazepam and its metabolites from hair samples. Analytical Chemistry, 79 (1). pp. 256-262. ISSN 0003-2700

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Abstract

An anti-diazepam, molecularly imprinted polymer (MIP) has been synthesized and used to extract diazepam and other benzodiazepines from hair samples via a molecularly imprinted solid-phase extraction (MISPE) protocol. Optimum retention of diazepam on the MIP columns was achieved using an apolar solvent, and the binding capacity of the polymer toward diazepam was found to be 110 ng of diazepam/mg of polymer. The recovery of a 50 ng diazepam standard spiked into blank hair was 93%, with good precision (RSD = 1.5%). The LOD and LOQ of diazepam in spiked hair samples were 0.09 and 0.14 ng/mg, respectively. The MISPE method was demonstrated to be applicable to the analysis of diazepam metabolites and other benzodiazepine drugs, in addition to diazepam itself. The application of the extraction method to postmortem hair samples yielded results that were in good agreement with the corresponding ELISA data (from blood samples) and data arising from the analysis of the same blood samples using a validated in-house SPE-LC−MS−MS method.

Item type: Article
ID code: 10246
Keywords: solid-phase extraction , diazepam , metabolites, Chemistry, Therapeutics. Pharmacology, Analytical Chemistry
Subjects: Science > Chemistry
Medicine > Therapeutics. Pharmacology
Department: Faculty of Science > Pure and Applied Chemistry
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 28 Nov 2011 12:15
    Last modified: 04 Sep 2014 20:46
    URI: http://strathprints.strath.ac.uk/id/eprint/10246

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